Technics for proclucture (Isolation) In natural heavy tide macrorganism usely grow in a camp mixed population cantining seural speics there for a singhle type of macrorgranism that can be adiquitely in a mixed calture . there for a need of proclucture is required that is ie population of cell arging singhle cell so there are three technices (1) spread plate (2) streak plate (3) pour plate (1) spread plate In a spread plate of a cell a mixture of an a agar surface so in to a completely separate colony (2) streak plate In this method the microbial mixture is transferred to the edge of and agar plate with the help of enunciation lop of and then stricked out over the surface in one of the suveral pattens (3) pour plate In this method in orginal several time to reduse microbial population saficiant to the seprate The small volume of several dilution are mixed with liquid agar that has been the mixture is poured emi