Skip to main content

Cultivation of Anerobic Bacteria

Cultivation of Anerobic Bacteria 


The main principle of cultivation anerobic bacteria is to reduse the oxygen cantaint of culture media and remove any oxygen which is already paresent the system in the medium

(1) Pre- Redused media   

For preparing a pre- reduced media the calcture media is bailed for several minute to derive of most of the oxygen content

oxygen free nitrogen is bubbled through the medium to keep it anerobic

The medium is then dispensed into tubes which are being flrshed with oxygen free nitrogen and are strelized by autoclaving

A reducing agent cystein is added to further lower

(2) Anaerobic chamber


This refer to a plastic anerobic glove box that contains an atmosphere of  H2  , CO2  & N2

culture media are placed with in the chamber by means of an air lock which can be evacuated by reaction with hydrogen forming water this reaction is aided by a paladium catalyst 

after being renderd oxygen free the media are inocculated with in the chamber are innoculated ( by means of the glove part ) and incubated with in the chamber 

(3)Anaerobic Jar 

It is a heavy walled jar with a gas tite seal whith in which tubes plate or other containrs to be incubated are pllaced along with H2 & Co2 generating system 

After the jar is shield oxygen present in the atmosphere inside the jar & dissolved in the  calture medium is gradually used up through reaction with the hydrogen in the presence of cataltst 

The air in the jar replaced with a mi2of H2 & Co2  thus  leading to anoxic condition 


Comments

Post a Comment

higherschool11.blogspot.com

Liver

Liver  The liver is the gland in the body weighing between 1 and 2.3 kg it is situated in  the upper part of the abdomonal cavity occupying the greater part of the right hypochondriac region part of the epigastric region and extending into the left hypochondriac region organs associated with the liverer The liver is endoclosed in a thin inelastic capsule and incompletely covered by a layer of peritoneumv. Folds of peritoneum form supporting ligaments atteching the liver to the inferior surface of the diaphragm  The liver has four lobes .The two most obivious are the large right lobe and the  smaller , wedge-shape left lobe .  The other two the caudate and quadrate lobes are areas on the posterior  The portal fissure This is the name given to the region on the posterior surface of the liver where various structure enter and leave the gland Blood supply  The hepatic aetery and the portal vein take blood to the liver venou...
Post a Comment
Read more

kidney

Kidney The kidneys are two bean-shaped organs in the renal system. They help the body pass waste as urine. They also help filter blood before sending it back to the heart. The kidneys perform many crucial functions, including: maintaining overall fluid balance regulating and filtering minerals from blood filtering waste materials from food, medications, and toxic substances creating hormones that help produce red blood cells, promote bone health, and regulate blood pressure Nephrons Nephrons are the most important part of each kidney. They take in blood, metabolize nutrients, and help pass out waste products from filtered blood. Each kidney has about 1 million nephrons. Each has its own internal set of structures. Renal corpuscle After blood enters a nephron, it goes into the renal corpuscle, also called a Malpighian body. The renal corpuscle contains two additional structures: The glomerulus.  This is a cluster of cap...
Post a Comment
Read more

Heart blood circulation

heart blood circulation  The circulatory system includes the  lymphatic system , which circulates  lymph . [1]  The passage of lymph for example takes much longer than that of blood. [2]  Blood is a fluid consisting of  plasma ,  red blood cells ,  white blood cells , and  platelets  that is circulated by the  heart  through the vertebrate vascular system, carrying oxygen and nutrients to and waste materials away from all body tissues. Lymph is essentially recycled excess blood plasma after it has been  filtered  from the  interstitial fluid  (between cells) and returned to the lymphatic system. The cardiovascular (from Latin words meaning "heart" and "vessel") system comprises the blood, heart, and  blood vessels . [3]  The lymph,  lymph nodes , and  lymph vessels  form the lymphatic system, which returns filtered blood plasma from the interstitial fluid (between cells...
Post a Comment
Read more